Shane Long has been forced to withdraw from Ireland's Euro 2020 qualifiers against Gibraltar and Georgia due to a groin injury.
The Southampton striker has been replaced in Mick McCarthy's group by Aiden O'Brien. The Millwall forward had been one of those cut from the 38-man provisional squad when McCarthy trimmed it to 23 last week.
It is the latest injury blow for Ireland ahead of the games.
On Saturday, Shamrock Rovers midfielder Jack Byrne was called up after Callum O'Dowda had been ruled out by a knee injury.
Just two days earlier, Josh Cullen and Kevin Long were both called up after injury also forced Alan Browne and Ronan Curtis to withdraw.
It leaves McCarthy's squad for the first games of his second term as follows:
Goalkeepers: Darren Randolph (Middlesbrough), Keiren Westwood (Sheffield Wednesday), Mark Travers (Bournemouth)
Defenders: Seamus Coleman (Everton), Matt Doherty (Wolverhampton Wanderers), Richard Keogh (Derby County), John Egan (Sheffield United), Shane Duffy (Brighton & Hove Albion), Enda Stevens (Sheffield United), Kevin Long (Burnley)
Midfielders: Alan Judge (Ipswich Town), Jeff Hendrick (Burnley), Conor Hourihane (Aston Villa), Harry Arter (Cardiff City*), Glenn Whelan (Aston Villa), Robbie Brady (Burnley), James McClean (Stoke City), Josh Cullen (Charlton*), Jack Byrne (Shamrock Rovers)
Forwards: Sean Maguire (Preston North End), David McGoldrick (Sheffield United), James Collins (Luton Town), Aiden O'Brien (Millwall)
Ireland play away to Gibraltar on Saturday and are then at home to Georgia on Tuesday.
Photo by Stephen McCarthy/Sportsfile